{"id":17952,"date":"2020-06-30T14:54:00","date_gmt":"2020-06-30T11:54:00","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/researchers-directly-obtain-3-d-full-color-images-with-conventional-microscope\/"},"modified":"2020-06-30T14:54:00","modified_gmt":"2020-06-30T11:54:00","slug":"researchers-directly-obtain-3-d-full-color-images-with-conventional-microscope","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/researchers-directly-obtain-3-d-full-color-images-with-conventional-microscope\/","title":{"rendered":"#Researchers directly obtain 3-D, full-color images with conventional microscope"},"content":{"rendered":"

“#Researchers directly obtain 3-D, full-color images with conventional microscope<\/strong>”<\/p>\n

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\n
\n
\"Researchers<\/img>
\n Imaging of a compound eye at different depths of (a) 14 \u03bcm and (b) 28 \u03bcm acquired with FC-WFM method, OS with FC-SIM method, and the OS in DOF with proposed FC-WFM-Deep, respectively. Credit: XIOM
\n <\/figcaption><\/figure>\n<\/div>\n<\/div>\n

Conventional wide-field microscopy (WFM) cannot provide optical sectioning (OS) images that are required for 3-D volumetric reconstruction. The reason lies in the fact that the out-of-focus signals are always coupled within the in-focus plane. By introducing structured illumination microscopy (SIM), researchers have achieved removing the out-of-focus components from the in-focus plane in full color (FC).<\/p>\n

\n <\/section>\n

However, the current FC-SIM app<\/a>roach needs three phase-shifted raw images for each focused plane and hundreds of axial-scanned planes. This approach causes a significant number of raw images and thus putting a heavy burden on data storage and processing time cost. How to release such a burden is still a question. <\/p>\n

Recently, a research team led by Prof. Yao Baoli at State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics (XIOPM) of the Chinese Academy of Science<\/a>s (CAS), has reported a deep learning scheme FC-WFM-Deep to obtain full-color optical sectioning images for wide-field microscopy directly.
\nIn contrast to the FC-SIM approach, the reconstruction data size is 21-fold smaller, and the in-focus depth is doubled. This work was published in the journal Biomedical Optics Express<\/i>.
\nFC-WFM-Deep fully exploits the unique high resolution and full-color capabilities of SIM. Efficiently, the deep learning network only needs training to a single wide-field frame. After training, high-quality images with optical sectioning, considerable in-focus depth, and full-color can be directly acquired from the wide-field frame.
\nFC-WFM-Deep has a comparable imaging quality to FC-SIM in terms of spatial resolution and dimensions. Beyond that, the data required for FC-WFM-Deep in full-color 3-D reconstruction can be 21-fold less than that for FC-SIM.
\nFC-WFM-Deep significantly reduces the 3-D data acquisition requirements without losing detail and improves the 3-D imaging speed by extracting the optical sectioning in the depth-of-field (DOF). This cost-effective and convenient method offers a promising tool to observe 3-D color biological samples with high precision.<\/p>\n

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More information:<\/strong>
\n Chen Bai et al. Full-color optically-sectioned imaging by wide-field microscopy via deep-learning, Biomedical Optics Express<\/i> (2020). DOI: 10.1364\/BOE.389852\n <\/div>\n

\n Citation<\/strong>:
\n Researchers directly obtain 3-D, full-color images with conventional microscope (2020, June 30)
\n retrieved 30 June 2020
\n from https:\/\/phys.org\/news<\/a>\/2020-06-d-full-color-images-conventional-microscope.html<\/p>\n

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\n part may be reproduced without the written permission. The content is provided for information purposes only.<\/p><\/div>\n<\/p><\/div>\n

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“#Researchers directly obtain 3-D, full-color images with conventional microscope” Imaging of a compound eye at different depths of (a) 14 \u03bcm and (b) 28 \u03bcm acquired with FC-WFM method, OS with FC-SIM method, and the OS in DOF with proposed FC-WFM-Deep, respectively. Credit: XIOM Conventional wide-field microscopy (WFM) cannot provide optical sectioning (OS) images that…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[29707,11724,29706],"class_list":["post-17952","post","type-post","status-publish","format-standard","hentry","category-sciencee","tag-full-color-images-with-conventional-microscope","tag-optics-photonics","tag-researchers-directly-obtain-3-d"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/17952","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=17952"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/17952\/revisions"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=17952"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=17952"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=17952"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}