{"id":21679,"date":"2020-07-06T17:50:00","date_gmt":"2020-07-06T14:50:00","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/structural-insights-into-fe-s-protein-biogenesis\/"},"modified":"2020-07-06T17:50:00","modified_gmt":"2020-07-06T14:50:00","slug":"structural-insights-into-fe-s-protein-biogenesis","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/structural-insights-into-fe-s-protein-biogenesis\/","title":{"rendered":"#Structural insights into Fe-S protein biogenesis"},"content":{"rendered":"

“#Structural insights into Fe-S protein biogenesis<\/strong>”<\/p>\n

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\"Structural<\/img>
\n Crystal structure of the CIA targeting complex provides insights into Fe-S protein biogenesis. Credit: Friedrich Miescher Institute for Biomedical Research
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The cytosolic iron sulfur assembly (CIA) pathway is required for the insertion of Fe-S clusters into proteins, including many DNA replication and repair factors. Despite its essential cellular function, this pathway remains enigmatic. A new integrative structural and biochemical study from the Thom\u00e4 group now provides detailed insights into the mechanisms of Fe-S protein biogenesis.<\/p>\n

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Iron-sulfur (Fe-S) clusters are small inorganic protein cofactors, which are important for protein stability and functionality. The most well known examples of Fe-S proteins are found in the respiratory chain of mitochondria. Fe-S clusters are also present in proteins involved in DNA replication and repair, and mutations in the Fe-S domains of DNA repair proteins are associated with hereditary disorders such as Trichothiodystrophy and Fanconi Anemia.<\/p>\n

The biogenesis of cytosolic and nuclear Fe-S proteins depends on a complex pathway known as the cytosolic iron sulfur assembly (CIA) pathway. This pathway is required for the initial assembly of the Fe-S clusters and for the transfer of the Fe-S clusters into client proteins. Despite its essential cellular function, this pathway is not well understood; in particular, the molecular mechanisms of client protein recognition and Fe-S cluster transfer remain unknown. Indeed, Fe-S proteins do not contain any shared sequence or structural motifs, which would represent an obvious recognition element for the Fe-S biogenesis machinery.
\nA new study led by Susanne Kassube, postdoc in the Thom\u00e4 group, now sheds light on the mechanism of Fe-S protein biogenesis. Thanks to an integrative structural and biochemical app<\/a>roach, Kassube uncovered the molecular architecture of the cytosolic Fe-S biogenesis machinery (CIA targeting complex, or CTC) at atomic resolution using X-ray crystallography, and visualized its interactions with two different client proteins using cryo-EM.
\n“Our structural and functional studies of the CTC visualize a major component of the CIA machinery and reveal a critical role of conformational dynamics of its largest subunit for client protein recognition, and potentially also Fe-S cluster transfer,” says Kassube. “Further research is needed to uncover the precise roles of the Fe-S clusters in DNA replication and repair, but we have made a big step towards understanding the cellular pathway that is crucial for their biogenesis.”<\/p>\n

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More information:<\/strong>
\n Susanne A. Kassube and Nicolas H. Thom\u00e4 (2020) Structural insights into Fe-S protein biogenesis by the CIA targeting complex. Nature Structural & Molecular Biology<\/i>, DOI: 10.1038\/s41594-020-0454-0 , www.nature.com\/articles\/s41594-020-0454-0\n <\/div>\n

\n Citation<\/strong>:
\n Structural insights into Fe-S protein biogenesis (2020, July 6)
\n retrieved 6 July 2020
\n from https:\/\/phys.org\/news<\/a>\/2020-07-insights-fe-s-protein-biogenesis.html<\/p>\n

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“#Structural insights into Fe-S protein biogenesis” Crystal structure of the CIA targeting complex provides insights into Fe-S protein biogenesis. Credit: Friedrich Miescher Institute for Biomedical Research The cytosolic iron sulfur assembly (CIA) pathway is required for the insertion of Fe-S clusters into proteins, including many DNA replication and repair factors. Despite its essential cellular function,…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[19221,33475],"class_list":["post-21679","post","type-post","status-publish","format-standard","hentry","category-sciencee","tag-molecular-computational-biology","tag-structural-insights-into-fe-s-protein-biogenesis"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/21679","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=21679"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/21679\/revisions"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=21679"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=21679"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=21679"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}