{"id":312211,"date":"2021-07-30T17:42:44","date_gmt":"2021-07-30T14:42:44","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/molecular-mechanism-of-ruthenium-complex-induced-dna-phase-separation-revealed\/"},"modified":"2021-07-30T17:42:44","modified_gmt":"2021-07-30T14:42:44","slug":"molecular-mechanism-of-ruthenium-complex-induced-dna-phase-separation-revealed","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/molecular-mechanism-of-ruthenium-complex-induced-dna-phase-separation-revealed\/","title":{"rendered":"#Molecular mechanism of ruthenium-complex-induced DNA phase separation revealed"},"content":{"rendered":"

“#Molecular mechanism of ruthenium-complex-induced DNA phase separation revealed<\/strong>”<\/p>\n

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\n The mechanisms of DNA phase separation induced by ruthenium complex. Credit: ZHANG Yuebin and LI Guohui
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The phenomenon of “liquid-liquid” phase separation (LLPS) of biological macromolecules in living cells regulates many cell activities.<\/p>\n


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DNA LLPS manipulates many important processes such as gene transcript<\/a>ion, translation, and chromosome high-level structure assembly. Abnormity of DNA LLPS causes oncogene expression, genome inactivation, uncontrolled transcription activation and other cellular processes, which are directly related to fatal diseases.<\/p>\n

Recently, a research group led by Prof. Li Guohui from the Dalian Institute of Chemical Physics (DICP) of the Chinese Academy of Science<\/a>s (CAS), in collaboration with Prof. Mao Zongwan’s group from Sun Yat-Sen University, revealed the molecular mechanism of ruthenium complex induced DNA phase separation in living cells.<\/p>\n

This study was published in Journal of the American Chemical Society<\/i> on July 22.<\/p>\n

Prof. Mao’s group proposed a metal ruthenium complex with high DNA affinity and photo-switching properties to fulfill the real-time detecting and monitoring of DNA phase separation process in living cells. In addition, the metal ruthenium complexes also exhibited potent anticancer activity both in Vitro and in Vivo conditions.<\/p>\n

Prof Li’s group utilized multi-scale molecular dynamics simulations to unveil the underlying mechanism of ruthenium complex induced DNA phase separation, where the positively charged lipophilic trip<\/a>henylphosphine substituents and the flexible long alkyl chains of the ruthenium complex provided significant contributions in inducing DNA assembly.<\/p>\n

This study provides new ideas for the design of interventional reagents for inducing DNA phase separation of living cells.<\/p>\n\n

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Research illuminates earliest part of phase separation\n <\/p><\/div>\n

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\n More information:<\/strong>
\n Wen-Jin Wang et al, Induction and Monitoring of DNA Phase Separation in Living Cells by a Light-Switching Ruthenium Complex, Journal of the American Chemical Society<\/i> (2021). DOI: 10.1021\/jacs.1c01424<\/a><\/p><\/div>\n
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\n Chinese Academy of Sciences
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Citation<\/strong>:
\n Molecular mechanism of ruthenium-complex-induced DNA phase separation revealed (2021, July 30)
\n retrieved 30 July 2021
\n from https:\/\/phys.org\/news<\/a>\/2021-07-molecular-mechanism-ruthenium-complex-induced-dna-phase.html<\/p>\n

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