{"id":322346,"date":"2021-08-11T22:35:26","date_gmt":"2021-08-11T19:35:26","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/new-technique-illuminates-dna-helix\/"},"modified":"2021-08-11T22:35:26","modified_gmt":"2021-08-11T19:35:26","slug":"new-technique-illuminates-dna-helix","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/new-technique-illuminates-dna-helix\/","title":{"rendered":"#New technique illuminates DNA helix"},"content":{"rendered":"

“#New technique illuminates DNA helix<\/strong>”<\/p>\n

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\"DNA\"
\n 3D-model of DNA. Credit: Michael Str\u00f6ck\/Wikimedia<\/a>\/ GNU Free Documentation License
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Cornell researchers have identified a new way to measure DNA torsional stiffness\u2014how much resistance the helix offers when twisted\u2014information that can potentially shed light on how cells work.<\/p>\n


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Understanding DNA is critically important: It stores the information that drives how cells work and is increasingly being used in nano- and biotechnology<\/a> app<\/a>lications. One key question for DNA researchers has been what role the helical nature of DNA plays in processes that take place on DNA.<\/p>\n

As a motor protein moves forward along DNA, it must twist or rotate the DNA, and therefore work against the torsional resistance of the DNA. (These motors can carry out gene expression or DNA replication as they move along DNA.) If a motor protein encounters too much resistance, it may stall. While scientists know that DNA torsional stiffness plays a crucial role in the fundamental processes of DNA, measuring torsional stiffness experimentally has been exceedingly difficult.<\/p>\n

In “Torsional Stiffness of Extended and Plectonemic DNA,” published July 7 in Physical Review Letters<\/i>, researchers report on a new way to measure DNA torsional stiffness by measuring how hard it is to twist the DNA when the DNA end-to-end distance is held constant.<\/p>\n

“We figured out a very clever trick to measure the torsional stiffness of DNA,” said senior author Michelle Wang, the James Gilbert White Distinguished Professor in the Physical Science<\/a>s in the Department of Physics in the College of Arts and Sciences and investigator of the Howard Hughes Medical Institute.<\/p>\n

“Intuitively, it seems that DNA will become extremely easy to twist under an extremely low force,” Wang said. “In fact, many people have made this assumption. We found that this is not the case, both experimentally and theoretically.”<\/p>\n

The first author is Xiang Gao, postdoctoral fellow in the Laboratory of Atomic and Solid State Physics.<\/p>\n

The technique also offers new opportunities to study twist-induced phase transitions in DNA and their biological implications. “Many colleagues commented to me that they were really excited about this finding as it has broad implications for DNA processes in vivo,” Wang said.<\/p>\n\n

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Scientists unwind mystery behind DNA replication\n <\/p><\/div>\n

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\n More information:<\/strong>
\n Xiang Gao et al, Torsional Stiffness of Extended and Plectonemic DNA, Physical Review Letters<\/i> (2021). DOI: 10.1103\/PhysRevLett.127.028101<\/a><\/p><\/div>\n
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\n Cornell University
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Citation<\/strong>:
\n New technique illuminates DNA helix (2021, August 11)
\n retrieved 11 August 2021
\n from https:\/\/phys.org\/news<\/a>\/2021-08-technique-illuminates-dna-helix.html<\/p>\n

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\n part may be reproduced without the written permission. The content is provided for information purposes only.<\/p><\/div>\n<\/p><\/div>\n