{"id":444065,"date":"2022-05-06T22:33:55","date_gmt":"2022-05-06T19:33:55","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/retinal-cell-map-could-advance-precise-therapies-for-blinding-diseases\/"},"modified":"2022-05-06T22:33:55","modified_gmt":"2022-05-06T19:33:55","slug":"retinal-cell-map-could-advance-precise-therapies-for-blinding-diseases","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/retinal-cell-map-could-advance-precise-therapies-for-blinding-diseases\/","title":{"rendered":"#Retinal cell map could advance precise therapies for blinding diseases"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a2e8eb0e9892\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a2e8eb0e9892\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/retinal-cell-map-could-advance-precise-therapies-for-blinding-diseases\/#%E2%80%9CRetinal_cell_map_could_advance_precise_therapies_for_blinding_diseases%E2%80%9D\" >&#8220;Retinal cell map could advance precise therapies for blinding diseases&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CRetinal_cell_map_could_advance_precise_therapies_for_blinding_diseases%E2%80%9D\"><\/span>&#8220;Retinal cell map could advance precise therapies for blinding diseases&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2022\/retinal-cell-map-could.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/retinal-cell-map-could.jpg\" data-sub-html=\"Using artificial intelligence, the researchers analyzed RPE from nine donor and identified five subpopulations of RPE. These populations are along a spectrum in terms of cell area, aspect ratio, hexagonality and number of neighbors. Foveal RPE (P1) required for central vision tend to be perfect hexagons and are tightly packed together. Peripheral RPE (P5) are less perfect hexagons and are more spread out. Credit: Davide Ortolan, Ph.D.\">\n<figure class=\"article-img\">\n            <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2022\/retinal-cell-map-could.jpg\" alt=\"Retinal cell map could advance precise therapies for blinding diseases\" title=\"Using artificial intelligence, the researchers analyzed RPE from nine donor and identified five subpopulations of RPE. These populations are along a spectrum in terms of cell area, aspect ratio, hexagonality and number of neighbors. Foveal RPE (P1) required for central vision tend to be perfect hexagons and are tightly packed together. Peripheral RPE (P5) are less perfect hexagons and are more spread out. Credit: Davide Ortolan, Ph.D.\" width=\"800\" height=\"454\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                Using artificial intelligence, the researchers analyzed RPE from nine donor and identified five subpopulations of RPE. These populations are along a spectrum in terms of cell area, aspect ratio, hexagonality and number of neighbors. Foveal RPE (P1) required for central vision tend to be perfect hexagons and are tightly packed together. Peripheral RPE (P5) are less perfect hexagons and are more spread out. Credit: Davide Ortolan, Ph.D.<br \/>\n            <\/figcaption><\/figure>\n<\/p><\/div>\n<\/div>\n<p>Researchers have identified distinct differences among the cells comprising a tissue in the retina that is vital to human visual perception. The scientists from the National Eye Institute (NEI) discovered five subpopulations of retinal pigment epithelium (RPE)\u2014a layer of tissue that nourishes and supports the retina&#8217;s light-sensing photoreceptors. Using artificial intelligence, the researchers analyzed images of RPE at single-cell resolution to create a reference map that locates each subpopulation within the eye. A report on the research published in <i>Proceedings of the National Academy of <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">Science<\/a>s<\/i>.\n                                                <\/p>\n<p>                                                                                &#8220;These results provide a first-of-its-kind framework for understanding different RPE cell subpopulations and their vulnerability to retinal diseases, and for developing targeted therapies to treat them,&#8221; said Michael F. Chiang, M.D., director of the NEI, part of the National Institutes of Health.<\/p>\n<p>&#8220;The findings will help us develop more precise cell and gene therapies for specific degenerative eye diseases,&#8221; said the study&#8217;s lead investigator, Kapil Bharti, Ph.D., who directs the NEI Ocular and Stem Cell Translational Research Section.<\/p>\n<p>Vision begins when light hits the rod and cone photoreceptors that line the retina in the back of the eye. Once activated, photoreceptors send signals through a complex network of other retinal neurons that converge at the optic nerve before <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/trip-and-travel\/\" data-internallinksmanager029f6b8e52c=\"10\" title=\"Trip &amp; Travel\" target=\"_blank\" rel=\"noopener\">travel<\/a>ing to various centers in the brain. The RPE sits beneath the photoreceptors as a monolayer, one cell deep.<\/p>\n<p>Age and disease can cause metabolic changes in RPE cells that can lead to photoreceptor degeneration. The impact on vision from these RPE changes varies dramatically by severity and where the RPE cells reside within the retina. For example, late-onset retinal degeneration (L-ORD) affects mostly peripheral retina and, therefore, peripheral vision. Age-related macular degeneration (AMD), a leading cause of vision loss, primarily affects RPE cells in the macula, which is crucial for central vision.<\/p>\n<p>Bharti and colleagues sought to determine if there are different RPE subpopulations that might explain the wide spectrum of retinal disease phenotypes.<\/p>\n<p>The team used artificial intelligence (AI) to analyze RPE cell morphometry, the external shape and dimensions of each cell. They trained a computer using fluorescently labelled images of RPE to analyze the entire human RPE monolayer from nine cadaver donors with no history of significant eye disease.<\/p>\n<figure class=\"mb-4\" itemscope=\"\" itemtype=\"http:\/\/schema.org\/VideoObject\">\n    <meta itemprop=\"name\" content=\"Retinal cell map could advance precise therapies for blinding diseases\"\/><br \/>\n    <meta itemprop=\"url\" content=\"https:\/\/scx2.b-cdn.net\/gfx\/video\/2022\/retinal-cell-map-could.mp4\"\/><br \/>\n    <meta itemprop=\"description\" content=\"Watch this video to learn more about RPE and what the single-cell resolution map shows. Credit: National Eye Institute\"\/><br \/>\n    <meta itemprop=\"uploadDate\" content=\"2022-05-06T15:33:35-04:00\"\/><br \/>\n        <meta itemprop=\"thumbnailUrl\" content=\"https:\/\/scx1.b-cdn.net\/gfx\/video_tmb\/2022\/retinal-cell-map-could.mp4.jpg\"\/><br \/>\n    <meta itemprop=\"contentUrl\" content=\"https:\/\/scx2.b-cdn.net\/gfx\/video\/2022\/retinal-cell-map-could.mp4\"\/><br \/>\n            <video class=\"embed-responsive embed-responsive-16by9\" id=\"jwVID69295\" controls=\"\" poster=\"https:\/\/scx1.b-cdn.net\/gfx\/video_tmb\/2022\/retinal-cell-map-could.mp4.jpg\"><source src=\"https:\/\/scx2.b-cdn.net\/gfx\/video\/2022\/retinal-cell-map-could.mp4\" type=\"video\/mp4\"><\/source><\/video><figcaption class=\"text-darken text-low-up mt-4\" itemprop=\"caption\">Watch this video to learn more about RPE and what the single-cell resolution map shows. Credit: National Eye Institute<\/figcaption><\/figure>\n<p>Morphometry features were calculated for each RPE cell\u2014on average, about 2.8 million cells per donor; 47.6 million cells were analyzed in total. The algorithm assessed each cell&#8217;s area, aspect ratio (width to height), hexagonality, and number of neighbors. Previous studies had suggested that RPE function is tied to the tightness of cellular junctions; the more crowded, the better for indicating cellular health.<br \/>\n                                            <!-- Google middle Adsense block --><\/p>\n<p>Based on morphometry, they identified five distinct RPE cell subpopulations, referred to as P1-P5, organized in concentric circles around the fovea, which is the center of the macula and the most light-sensitive region of the retina. Compared to RPE in the periphery, foveal RPE tend to be perfectly hexagonal and more compactly situated, with higher numbers of neighboring cells.<\/p>\n<p>Unexpectedly, they discovered that the peripheral retina contains a ring of RPE cells (P4) with a cell area very similar to RPE in and around the macula.<\/p>\n<p>&#8220;The presence of the P4 subpopulation highlights the diversity within retinal periphery, suggesting that there could be functional differences among RPE that we are currently unaware of,&#8221; said the study&#8217;s first author, Davide Ortolan, Ph.D. a research fellow in the NEI Ocular and Stem Cell Translational Research Section. &#8220;Future studies are needed to help us understand the role of this subpopulation.&#8221;<\/p>\n<p>Next, they analyzed RPE from cadavers with AMD. Foveal (P1) RPE tended to be absent due to disease damage, and the differences among cells in the P2-P5 subpopulations were not statistically significant. Overall, the AMD RPE subpopulations tended to be elongated relative to RPE cells not affected by AMD.<\/p>\n<p>To further test the hypothesis that different retinal degenerations affect specific RPE subpopulations, they analyzed ultrawide-field fundus autofluorescence images from patients affected by choroideremia, L-ORD, or a retinal degeneration with no identified molecular cause. While these studies were conducted at a single point in time, they still demonstrated that different RPE subpopulations are vulnerable to different types of retinal degenerative diseases.<\/p>\n<p>&#8220;Overall, the results suggest that AI can detect changes of RPE cell morphometry prior to the development of visibly <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">app<\/a>arent degeneration,&#8221; said Ortolan.<\/p>\n<p>Age-related morphometric changes also may appear in some RPE subpopulations before they&#8217;re detectable in others. These finding will help inform future studies using noninvasive imaging technologies, such as adaptive optics, which resolve retinal cells in unprecedented detail and could potentially be used to predict changes in RPE health in living patients.\n                                                                                                                        <\/p>\n<hr\/>\n<div class=\"article-main__explore my-4 d-print-none\">\n<p>                                                                                        Improved retinal transplant technique ready for clinical trials\n                                                                                    <\/p><\/div>\n<hr class=\"mb-4\"\/>\n<div class=\"article-main__more p-4\">\n                                                                                                <strong>More information:<\/strong><br \/>\n                                                Davide Ortolan et al, Single-cell\u2013resolution map of human retinal pigment epithelium helps discover subpopulations with differential disease sensitivity, <i>Proceedings of the National Academy of Sciences<\/i> (2022).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1073\/pnas.2117553119\">DOI: 10.1073\/pnas.2117553119<\/a><\/p><\/div>\n<div class=\"d-inline-block text-medium my-4\">\n                                                Provided by<br \/>\n                                                                                                    National Eye Institute<br \/>\n                                                                                                        <a rel=\"nofollow noopener\" target=\"_blank\" class=\"icon_open\" href=\"http:\/\/www.nei.nih.gov\/\"><br \/>\n                                                        <svg>\n                                                            <use href=\"https:\/\/medx.b-cdn.net\/tmpl\/v6\/img\/svg\/sprite.svg#icon_open\" x=\"0\" y=\"0\"\/>\n                                                        <\/svg><br \/>\n                                                    <\/a><\/p><\/div>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>\n                                                 <strong>Citation<\/strong>:<br \/>\n                                                 Retinal cell map could advance precise therapies for blinding diseases (2022, May  6)<br \/>\n                                                 retrieved  9 May 2022<br \/>\n                                                 from https:\/\/medicalxpress.com\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2022-05-retinal-cell-advance-precise-therapies.html<\/p>\n<p>                                            This document is subject to copyright. 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These populations are along a spectrum in terms of cell area, aspect ratio, hexagonality and number of neighbors. Foveal RPE (P1) required for central vision tend to be perfect&#8230;<\/p>\n","protected":false},"author":1,"featured_media":444066,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/retinal-cell-map-could.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-444065","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/444065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=444065"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/444065\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/444066"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=444065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=444065"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=444065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}