{"id":460056,"date":"2022-06-09T16:56:03","date_gmt":"2022-06-09T13:56:03","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/newly-discovered-molecular-network-regulates-vitamin-c-biosynthesis-in-kiwifruit\/"},"modified":"2022-06-09T16:56:03","modified_gmt":"2022-06-09T13:56:03","slug":"newly-discovered-molecular-network-regulates-vitamin-c-biosynthesis-in-kiwifruit","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/newly-discovered-molecular-network-regulates-vitamin-c-biosynthesis-in-kiwifruit\/","title":{"rendered":"#Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_85 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a5064130cc78\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a5064130cc78\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/newly-discovered-molecular-network-regulates-vitamin-c-biosynthesis-in-kiwifruit\/#%E2%80%9CNewly_discovered_molecular_network_regulates_vitamin_C_biosynthesis_in_kiwifruit%E2%80%9D\" >&#8220;Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CNewly_discovered_molecular_network_regulates_vitamin_C_biosynthesis_in_kiwifruit%E2%80%9D\"><\/span>&#8220;Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2022\/newly-discovered-molec.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/2022\/newly-discovered-molec.jpg\" data-sub-html=\"The circle bar charts showing the AsA content (log2) of 48 different Actinidia species. Credit: WBG\">\n<figure class=\"article-img\">\n            <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2022\/newly-discovered-molec.jpg\" alt=\"Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit\" title=\"The circle bar charts showing the AsA content (log2) of 48 different Actinidia species. Credit: WBG\" width=\"800\" height=\"530\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                The circle bar charts showing the AsA content (log2) of 48 different Actinidia species. Credit: WBG<br \/>\n            <\/figcaption><\/figure>\n<\/p><\/div>\n<\/div>\n<p>Actinidia (kiwifruit) is rich in ascorbic acid (AsA) and Vitamin C (Vc) and is known as the &#8220;King of Vc,&#8221; which makes it very popular with consumers. Interestingly, the fruit AsA content of different kiwifruit species varies up to 300- to 400-fold. However, the molecular regulation mechanisms of AsA synthesis in kiwifruit are still poorly understood.<\/p>\n<section class=\"article-banner first-banner ads-336x280\">\n         <!-- \/4988204\/Phys_Story_InText_Box --><\/p>\n<\/section>\n<p>Supervised by Prof. Zhong Caihong and Associate Prof. Li Dawei, Ph.D. student Liu Xiaoying from the Wuhan Botanical Garden of the Chinese Academy of <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">Science<\/a>s first carried out systematic measurements of fruit AsA content in 48 kiwifruit species and found that the differences in AsA between kiwifruit species were mainly due to the extent of AsA accumulation in the early fruit development.<\/p>\n<p>In addition, RNA-Seq, transient transformation, transgenic and gene editing were performed on kiwifruit with a high AsA content (six to ten-fold that of commercial kiwifruit) to uncover AceGGP3, a key gene for AsA synthesis in Actinidia eriantha (A. eriantha).<\/p>\n<p>They identified a 1R-subtype myeloblastosis (MYB)-type tran<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">script<\/a>ion factor, AceMYBS1 by transcriptome analysis and RT-qPCR. Overexpression editing of AceMYBS1 in A. eriantha showed that AceMYBS1 promoted AsA accumulation.<\/p>\n<p>Subsequently, yeast 1-hybrid, dual-luciferase assay and electrophoretic mobility shift assay revealed that AceMYBS1 bound directly to the AceGGP3 promoter and activated transcription of the AceGGP3 gene, increasing AsA accumulation.<\/p>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2022\/newly-discovered-molec-1.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/newly-discovered-molec-1.jpg\" data-sub-html=\"Circle heatmap showing the expression profifile (log2FPKM) of orthologous genes in the L-galactose pathway in the kiwifruit. Credit: WBG\">\n<figure class=\"article-img text-center\">\n            <img decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2022\/newly-discovered-molec-1.jpg\" alt=\"Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit\" title=\"Circle heatmap showing the expression profifile (log2FPKM) of orthologous genes in the L-galactose pathway in the kiwifruit. Credit: WBG\"\/><figcaption class=\"text-left text-darken text-truncate text-low-up mt-3\">\n                Circle heatmap showing the expression profifile (log2FPKM) of orthologous genes in the L-galactose pathway in the kiwifruit. Credit: WBG<br \/>\n            <\/figcaption><\/figure>\n<\/p><\/div>\n<\/div>\n<p>Further, they uncovered the downstream protein AceGBF3 (a G-box binding factor) that interacted with AceMYBS1 using yeast 2-hybrid library screening assay. Additionally, yeast-2 hybrid, bimolecular luminescence complementation assay, pull-down, bimolecular fluorescence complementation assay and transgenic assay were carried out to demonstrate that AceMYBS1 interacted with AceGBF3 in vitro and in vivo.<\/p>\n<p>Moreover, the interaction between AceMYBS1 and AceGBF3 can activate the transcription of AceGGP3 and synergistically promote the AsA synthesis of A. eriantha. <\/p>\n<p>Previous studies have suggested that hormones could affect AsA synthesis. The newly-discovered AceGBF3-AceMYBS1-AceGGP3 molecular regulatory network by Zhong&#8217;s team can also be inhibited by abscisic acid, which is a foundation that will provide new clues for future elucidation of hormonal regulation of Vitamin C synthesis.<\/p>\n<p>The study has been published in <i>New Phytologist<\/i>, titled &#8220;Kiwifruit MYBS1-like and GBF3 transcription factors influence L-ascorbic acid biosynthesis by activating transcription of GDP-L-galactose phosphorylase 3.&#8221;<\/p>\n<hr\/>\n<div class=\"article-main__explore my-4 d-print-none\">\n<p>                                            Novel transcription factor inhibits flavone and anthocyanin biosynthesis in chrysanthemum at high temperatures\n                                        <\/p><\/div>\n<hr class=\"mb-4\"\/>\n<div class=\"article-main__more p-4\">\n                                                                                                <strong>More information:<\/strong><br \/>\n                                                Xiaoying Liu et al, Kiwifruit MYBS1\u2010like and GBF3 transcription factors influence l \u2010ascorbic acid biosynthesis by activating transcription of GDP\u2010L\u2010galactose phosphorylase 3, <i>New Phytologist<\/i> (2022).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1111\/nph.18097\">DOI: 10.1111\/nph.18097<\/a><\/p><\/div>\n<div class=\"d-inline-block text-medium my-4\">\n                                                Provided by<br \/>\n                                                                                                    Chinese Academy of Sciences<br \/>\n                                                                                                        <a rel=\"nofollow noopener\" target=\"_blank\" class=\"icon_open\" href=\"https:\/\/english.cas.cn\/\"><br \/>\n                                                        <svg>\n                                                            <use href=\"https:\/\/phys.b-cdn.net\/tmpl\/v6\/img\/svg\/sprite.svg#icon_open\" x=\"0\" y=\"0\"\/>\n                                                        <\/svg><br \/>\n                                                    <\/a><\/p><\/div>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>                                                 <strong>Citation<\/strong>:<br \/>\n                                                 Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit (2022, June  9)<br \/>\n                                                 retrieved  9 June 2022<br \/>\n                                                 from https:\/\/phys.org\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2022-06-newly-molecular-network-vitamin-biosynthesis.html<\/p>\n<p>                                            This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no<br \/>\n                                            part may be reproduced without the written permission. The content is provided for information purposes only.<\/p><\/div>\n<\/p><\/div>\n<p><script id=\"facebook-jssdk\" async=\"\" src=\"https:\/\/connect.facebook.net\/en_US\/sdk.js\"><\/script><\/p>\n<blockquote><p><strong><span style=\"color: #ff6600;\">If you liked the article, do not forget to share it with your friends. Follow us on\u00a0<span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"https:\/\/news.google.com\/publications\/CAAqBwgKMLG0nwswvr63Aw\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">Google News<\/a><\/span>\u00a0too, click on the star and choose us from your favorites.<\/span><\/strong><\/p><\/blockquote>\n<blockquote>\n<p style=\"text-align: center;\">For forums sites go to <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/forum.buradabiliyorum.com\/\" target=\"_blank\" rel=\"noopener\">Forum.BuradaBiliyorum.Com<\/a><\/span><\/strong>\n<\/p><\/blockquote>\n<blockquote>\n<p style=\"text-align: center;\"><strong>If you want to read more Like this articles, you can visit our <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/en.buradabiliyorum.com\/science\/\" target=\"_blank\" rel=\"noopener\">Science category.<\/a><\/span><\/strong><\/p>\n<\/blockquote>\n<p><span style=\"color: black;\"><a style=\"color: #ff9900;\" href=\"https:\/\/phys.org\/news\/2022-06-newly-molecular-network-vitamin-biosynthesis.html\" target=\"_blank\" rel=\"noopener\">Source<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;Newly discovered molecular network regulates vitamin C biosynthesis in kiwifruit&#8221; The circle bar charts showing the AsA content (log2) of 48 different Actinidia species. Credit: WBG Actinidia (kiwifruit) is rich in ascorbic acid (AsA) and Vitamin C (Vc) and is known as the &#8220;King of Vc,&#8221; which makes it very popular with consumers. Interestingly, the&#8230;<\/p>\n","protected":false},"author":1,"featured_media":460057,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/2022\/newly-discovered-molec.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-460056","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/460056","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=460056"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/460056\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/460057"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=460056"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=460056"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=460056"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}