{"id":516777,"date":"2022-11-26T18:10:02","date_gmt":"2022-11-26T15:10:02","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/some-archaea-found-to-have-integrons-allowing-cross-domain-gene-transfer\/"},"modified":"2022-11-26T18:10:02","modified_gmt":"2022-11-26T15:10:02","slug":"some-archaea-found-to-have-integrons-allowing-cross-domain-gene-transfer","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/some-archaea-found-to-have-integrons-allowing-cross-domain-gene-transfer\/","title":{"rendered":"#Some Archaea found to have integrons, allowing cross-domain gene transfer"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_85 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a3be4512bcef\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a3be4512bcef\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/some-archaea-found-to-have-integrons-allowing-cross-domain-gene-transfer\/#%E2%80%9CSome_Archaea_found_to_have_integrons_allowing_cross-domain_gene_transfer%E2%80%9D\" >&#8220;Some Archaea found to have integrons, allowing cross-domain gene transfer&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CSome_Archaea_found_to_have_integrons_allowing_cross-domain_gene_transfer%E2%80%9D\"><\/span>&#8220;Some Archaea found to have integrons, allowing cross-domain gene transfer&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2022\/some-archaea-found-to.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/some-archaea-found-to.jpg\" data-sub-html=\"Cassette recruitment (&lt;i&gt;attC&lt;\/i&gt; \u00d7 &lt;i&gt;attI&lt;\/i&gt; recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (Km&lt;sup&gt;R&lt;\/sup&gt;) suicide vector pJP5603 with an &lt;i&gt;attC&lt;\/i&gt; site is delivered into the recipient &lt;i&gt;E. coli&lt;\/i&gt; UB5201 strain via conjugation. The recipient strain carries an &lt;i&gt;intI1&lt;\/i&gt; gene, expressed from the inducible P&lt;sub&gt;BAD&lt;\/sub&gt; promoter, and an &lt;i&gt;attI1&lt;\/i&gt; site, residing on the carbenicillin resistance (Cb&lt;sup&gt;R&lt;\/sup&gt;) pBAD24 and chloramphenicol resistance (Cm&lt;sup&gt;R&lt;\/sup&gt;) pACYC184 backbones, respectively. The donor suicide vector cannot replicate within the recipient host and thus can only persist following &lt;i&gt;attC&lt;\/i&gt; \u00d7 &lt;i&gt;attI&lt;\/i&gt; recombination to form a plasmid cointegrate. (B) Average recombination frequencies (log&lt;sub&gt;10&lt;\/sub&gt; scale, \u00b11\u00a0SE) between &lt;i&gt;attI1&lt;\/i&gt; and nine archaeal &lt;i&gt;attCs&lt;\/i&gt; (with phyla of origin labeled along the x axis) and the paradigmatic bacterial &lt;i&gt;attC&lt;\/i&gt; site (&lt;i&gt;attC&lt;sub&gt;aadA7&lt;\/sub&gt;&lt;\/i&gt;), used as positive control. Average frequencies were calculated following three independent cassette insertion assays (see Materials and Methods for details). No statistically significant difference in recombination frequencies were detected among the tested &lt;i&gt;attCs&lt;\/i&gt; (Kruskal-Wallis test, n\u00a0=\u00a027; df\u00a0=\u00a08, P =\u00a00.488). Recombination frequencies are shown for &lt;i&gt;attC&lt;\/i&gt; bottom strands only. See table S1 for &lt;i&gt;attC&lt;\/i&gt; top strand recombination frequencies. N.S., not significant. Credit: &lt;i&gt;Science Advances&lt;\/i&gt; (2022). DOI: 10.1126\/sciadv.abq6376\">\n<figure class=\"article-img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2022\/some-archaea-found-to.jpg\" alt=\"Some archaea found to have integrons, allowing cross-domain gene transfer\" title=\"Cassette recruitment (&lt;i&gt;attC&lt;\/i&gt; \u00d7 &lt;i&gt;attI&lt;\/i&gt; recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (Km&lt;sup&gt;R&lt;\/sup&gt;) suicide vector pJP5603 with an &lt;i&gt;attC&lt;\/i&gt; site is delivered into the recipient &lt;i&gt;E. coli&lt;\/i&gt; UB5201 strain via conjugation. The recipient strain carries an &lt;i&gt;intI1&lt;\/i&gt; gene, expressed from the inducible P&lt;sub&gt;BAD&lt;\/sub&gt; promoter, and an &lt;i&gt;attI1&lt;\/i&gt; site, residing on the carbenicillin resistance (Cb&lt;sup&gt;R&lt;\/sup&gt;) pBAD24 and chloramphenicol resistance (Cm&lt;sup&gt;R&lt;\/sup&gt;) pACYC184 backbones, respectively. The donor suicide vector cannot replicate within the recipient host and thus can only persist following &lt;i&gt;attC&lt;\/i&gt; \u00d7 &lt;i&gt;attI&lt;\/i&gt; recombination to form a plasmid cointegrate. (B) Average recombination frequencies (log&lt;sub&gt;10&lt;\/sub&gt; scale, \u00b11\u00a0SE) between &lt;i&gt;attI1&lt;\/i&gt; and nine archaeal &lt;i&gt;attCs&lt;\/i&gt; (with phyla of origin labeled along the x axis) and the paradigmatic bacterial &lt;i&gt;attC&lt;\/i&gt; site (&lt;i&gt;attC&lt;sub&gt;aadA7&lt;\/sub&gt;&lt;\/i&gt;), used as positive control. Average frequencies were calculated following three independent cassette insertion assays (see Materials and Methods for details). No statistically significant difference in recombination frequencies were detected among the tested &lt;i&gt;attCs&lt;\/i&gt; (Kruskal-Wallis test, n\u00a0=\u00a027; df\u00a0=\u00a08, P =\u00a00.488). Recombination frequencies are shown for &lt;i&gt;attC&lt;\/i&gt; bottom strands only. See table S1 for &lt;i&gt;attC&lt;\/i&gt; top strand recombination frequencies. N.S., not significant. Credit: &lt;i&gt;Science Advances&lt;\/i&gt; (2022). DOI: 10.1126\/sciadv.abq6376\" width=\"800\" height=\"440\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                Cassette recruitment (<i>attC<\/i> \u00d7 <i>attI<\/i> recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (Km<sup>R<\/sup>) suicide vector pJP5603 with an <i>attC<\/i> site is delivered into the recipient <i>E. coli<\/i> UB5201 strain via conjugation. The recipient strain carries an <i>intI1<\/i> gene, expressed from the inducible P<sub>BAD<\/sub> promoter, and an <i>attI1<\/i> site, residing on the carbenicillin resistance (Cb<sup>R<\/sup>) pBAD24 and chloramphenicol resistance (Cm<sup>R<\/sup>) pACYC184 backbones, respectively. The donor suicide vector cannot replicate within the recipient host and thus can only persist following <i>attC<\/i> \u00d7 <i>attI<\/i> recombination to form a plasmid cointegrate. (B) Average recombination frequencies (log<sub>10<\/sub> scale, \u00b11\u00a0SE) between <i>attI1<\/i> and nine archaeal <i>attCs<\/i> (with phyla of origin labeled along the x axis) and the paradigmatic bacterial <i>attC<\/i> site (<i>attC<sub>aadA7<\/sub><\/i>), used as positive control. Average frequencies were calculated following three independent cassette insertion assays (see Materials and Methods for details). No statistically significant difference in recombination frequencies were detected among the tested <i>attCs<\/i> (Kruskal-Wallis test, n\u00a0=\u00a027; df\u00a0=\u00a08, P =\u00a00.488). Recombination frequencies are shown for <i>attC<\/i> bottom strands only. See table S1 for <i>attC<\/i> top strand recombination frequencies. N.S., not significant. Credit: <i><a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">Science<\/a> Advances<\/i> (2022). DOI: 10.1126\/sciadv.abq6376<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>A team of researchers at Macquarie University, in Australia, has found evidence showing that some Archaea have integrons. In their paper published in the journal <i>Science Advances<\/i>, the group describes how they used a recently developed technique called metagenome-assembled genomes (MAG) to study the genomes of Archaea samples in a new way, and what they learned by doing so.<\/p>\n<section class=\"article-banner first-banner ads-336x280\"><!-- \/4988204\/Phys_Story_InText_Box --><br \/>\n      <\/section>\n<p>Life on Earth is divided into three domains, Eukarya, Bacteria and Archaea. The third domain, Archaea, is similar to Bacteria\u2014its members are often called Archaebacteria. Like Bacteria, Archaea are single-celled but unlike Bacteria, they rely on lipids in their cell membranes. <\/p>\n<p>In this new effort, the researchers were looking into the means by which Bacteria and Archaea swap genes and wondered if it was possible that they have integrons\u2014gene capture and dissemination systems in Bacteria that use gene cassettes to pass the proteins involved. To find out, they turned to MAG\u2014a technique that allows for searching for single gene and gene recombination sites called AttC that sequence for coding the protein integron integrase (IntI). <\/p>\n<p>Using the technique, they found many matches. Of 6,700 genomes scanned, they found 75 matches, across nine phyla, each evidence of an integron. And all of them turned out to have the same structure as the integrons found in Bacteria, which suggested the use of cassettes. <\/p>\n<p>The researchers believed that this indicated that the Archaea they identified should be able to swap genes with Bacteria and vice-versa, as easily as Bacteria swap genes among themselves. To prove that their idea was correct, they synthesized AttC from an Archaea specimen and exposed it to an E. coli specimen. Testing showed that cassettes had been created allowing gene sw<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">app<\/a>ing to occur.<\/p>\n<p>Finding integrons in Archaea will most certainly open up new avenues of research. One routed would be to look into the possibility that swapping genes from Archaea to Bacteria help the latter to become resistant to drugs meant to kill them. The researchers also note that it would be helpful if a complete genome of Archaea were made available.<\/p>\n<div class=\"article-main__more p-4\">\n                                                                                        <strong>More information:<\/strong><br \/>\n                                                Timothy M. Ghaly et al, Discovery of integrons in Archaea: Platforms for cross-domain gene transfer, <i>Science Advances<\/i> (2022).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1126\/sciadv.abq6376\">DOI: 10.1126\/sciadv.abq6376<\/a><\/p><\/div>\n<p class=\"article-main__note mt-4\">\n                                                  \u00a9 2022 Science X Network<\/p>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>                                                    <strong>Citation<\/strong>:<br \/>\n                                                    Some Archaea found to have integrons, allowing cross-domain gene transfer (2022, November 26)<br \/>\n                                                    retrieved 27 November 2022<br \/>\n                                                    from https:\/\/phys.org\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2022-11-archaea-integrons-cross-domain-gene.html<\/p>\n<p>                                             This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no<br \/>\n                                             part may be reproduced without the written permission. The content is provided for information purposes only.<\/p><\/div>\n<\/p><\/div>\n<p><script id=\"facebook-jssdk\" async=\"\" src=\"https:\/\/connect.facebook.net\/en_US\/sdk.js\"><\/script><\/p>\n<blockquote><p><strong><span style=\"color: #ff6600;\">If you liked the article, do not forget to share it with your friends. Follow us on\u00a0<span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"https:\/\/news.google.com\/publications\/CAAqBwgKMLG0nwswvr63Aw\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">Google News<\/a><\/span>\u00a0too, click on the star and choose us from your favorites.<\/span><\/strong><\/p><\/blockquote>\n<blockquote>\n<p style=\"text-align: center;\">For forums sites go to <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/forum.buradabiliyorum.com\/\" target=\"_blank\" rel=\"noopener\">Forum.BuradaBiliyorum.Com<\/a><\/span><\/strong><\/p>\n<\/blockquote>\n<blockquote>\n<p style=\"text-align: center;\"><strong>If you want to read more Like this articles, you can visit our <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/en.buradabiliyorum.com\/science\/\" target=\"_blank\" rel=\"noopener\">Science category.<\/a><\/span><\/strong><\/p>\n<\/blockquote>\n<p><span style=\"color: black;\"><a style=\"color: #ff9900;\" href=\"https:\/\/phys.org\/news\/2022-11-archaea-integrons-cross-domain-gene.html\" target=\"_blank\" rel=\"noopener\">Source<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;Some Archaea found to have integrons, allowing cross-domain gene transfer&#8221; Cassette recruitment (attC \u00d7 attI recombination) assays. (A) Schematic outlining the experimental setup of the cassette insertion assays. The kanamycin resistance (KmR) suicide vector pJP5603 with an attC site is delivered into the recipient E. coli UB5201 strain via conjugation. The recipient strain carries an&#8230;<\/p>\n","protected":false},"author":1,"featured_media":516778,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/some-archaea-found-to.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-516777","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/516777","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=516777"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/516777\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/516778"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=516777"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=516777"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=516777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}