{"id":517895,"date":"2022-11-29T14:04:04","date_gmt":"2022-11-29T11:04:04","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/bioreactor-keeps-cell-culture-conditions-under-control\/"},"modified":"2022-11-29T14:04:04","modified_gmt":"2022-11-29T11:04:04","slug":"bioreactor-keeps-cell-culture-conditions-under-control","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/bioreactor-keeps-cell-culture-conditions-under-control\/","title":{"rendered":"#Bioreactor keeps cell culture conditions under control"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a2c2a23b1876\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a2c2a23b1876\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/bioreactor-keeps-cell-culture-conditions-under-control\/#%E2%80%9CBioreactor_keeps_cell_culture_conditions_under_control%E2%80%9D\" >&#8220;Bioreactor keeps cell culture conditions under control&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CBioreactor_keeps_cell_culture_conditions_under_control%E2%80%9D\"><\/span>&#8220;Bioreactor keeps cell culture conditions under control&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2022\/bioreactor-keeps-cell-1.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/bioreactor-keeps-cell-1.jpg\" data-sub-html=\"A KAUST team of marine scientists and bioscientists has developed a cell-culturing technique that could lead to more accurate, interpretable and reproducible experiments in biomedical research. Credit: \u00a9 2022 KAUST; Anastasia Serin\">\n<figure class=\"article-img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2022\/bioreactor-keeps-cell-1.jpg\" alt=\"Bioreactor keeps cell culture conditions under control\" title=\"A KAUST team of marine scientists and bioscientists has developed a cell-culturing technique that could lead to more accurate, interpretable and reproducible experiments in biomedical research. Credit: \u00a9 2022 KAUST; Anastasia Serin\" width=\"800\" height=\"530\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                A KAUST team of marine scientists and bioscientists has developed a cell-culturing technique that could lead to more accurate, interpretable and reproducible experiments in biomedical research. Credit: \u00a9 2022 KAUST; Anastasia Serin<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>A cell-culturing technique developed by KAUST scientists helps to create biological conditions that more closely mirror physiological environments compared to standard protocols used in most laboratories today.<\/p>\n<section class=\"article-banner first-banner ads-336x280\"><!-- \/4988204\/Phys_Story_InText_Box --><br \/>\n      <\/section>\n<p>The new bioreactor system delivers gases\u2014rather than chemicals\u2014to keep acidity levels and oxygen exchange kinetics within body-like ranges, an <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">app<\/a>roach that allows for more careful control of environmental parameters.<\/p>\n<p>It is also more responsive to the metabolic activity of cells and more representative of what happens inside living organisms. If widely adopted, the platform could lead to more accurate, interpretable and reproducible experiments in biomedical research.<\/p>\n<p>The cell-culturing apparatus came about through a unique interdisciplinary collaboration between marine biologist Carlos Duarte and stem cell biologist Mo Li.<\/p>\n<p>Duarte and Li joined forces in recent years to highlight widespread problems in the control, monitoring and reporting of environmental conditions in cell-culturing systems. Together with their lab group members, including research scientist Shannon Klein and Ph.D. students Samhan Alsolami and Silvia Arossa, in 2022 they showed that common techniques for maintaining human pluripotent stem cells consistently produce large swings in ambient gas levels, pH and other parameters, changes that can lead to unpredictable shifts in the kinetics of cell growth.<\/p>\n<p>Following that finding, the researchers now describe how even small perturbations in pH can prompt human cells to dramatically reorient their gene expression profiles. &#8220;We revealed robust and coordinated changes in expression of genes related to inflammation and metabolism in response to acidic environments,&#8221; says Li. <\/p>\n<p>This result, outlined in their latest report, &#8220;emphasizes the importance of maintaining physiological conditions to avoid artifacts in research derived from human cell cultures,&#8221; Duarte says.<\/p>\n<p>Seeking to purge those artifacts from experiments and create a more stable culturing milieu, the team then retrofitted a standard bioreactor system with automated gas monitoring systems and valves for delivering pure inputs of oxygen, carbon dioxide and nitrogen.<\/p>\n<p>This allowed the researchers to maintain precise and physiologically relevant control over pH and other culture parameters, without having to rely on artificial buffering agents that have more limited capacity to preserve environmental stability.<\/p>\n<p>Notably, much of the inspiration for the experimental set-up came from Duarte&#8217;s previous investigations of marine ecosystems. Duarte had devised ways of fine-tuning pH and gas fluctuations in order to study the effects of ocean acidification on seagrass meadows in the Red Sea.<\/p>\n<p>As he explains: &#8220;We then used our experience in the accurate control of pH, O<sub>2<\/sub> and CO<sub>2<\/sub> in experiments with marine organisms to design systems that could achieve tight controls of the environment in human cell culture studies.&#8221;<\/p>\n<p>&#8220;It was a beautiful cross-fertilization between marine <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">science<\/a> and biomedical research,&#8221; Duarte says.<\/p>\n<div class=\"article-main__more p-4\">\n                                                                                        <strong>More information:<\/strong><br \/>\n                                                Silvia Arossa et al, A Gas-only Bioreactor System Maintains Stable Culture Environments and Reveals that Moderate pH deviations Trigger Transcriptome-wide Responses in Human Cells Cultured in Physioxia and Physiological Buffers, <i>Life Medicine<\/i> (2022).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1093\/lifemedi\/lnac056\">DOI: 10.1093\/lifemedi\/lnac056<\/a><\/p>\n<p> Shannon G. Klein et al, In situ monitoring reveals cellular environmental instabilities in human pluripotent stem cell culture, <i>Communications Biology<\/i> (2022).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1038\/s42003-022-03065-w\">DOI: 10.1038\/s42003-022-03065-w<\/a><\/p><\/div>\n<div class=\"d-inline-block text-medium mt-4\">\n<p>                                                    Provided by<br \/>\n                                                                                                            King Abdullah University of Science and <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/technology\/\" data-internallinksmanager029f6b8e52c=\"4\" title=\"Technology\" target=\"_blank\" rel=\"noopener\">Technology<\/a><br \/>\n                                                                                                                <a rel=\"nofollow noopener\" target=\"_blank\" class=\"icon_open\" href=\"https:\/\/www.kaust.edu.sa\/en\"><br \/>\n                                                            <svg><use href=\"https:\/\/phys.b-cdn.net\/tmpl\/v6\/img\/svg\/sprite.svg#icon_open\" x=\"0\" y=\"0\"\/><\/svg><\/a><\/p><\/div>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>                                                    <strong>Citation<\/strong>:<br \/>\n                                                    Bioreactor keeps cell culture conditions under control (2022, November 29)<br \/>\n                                                    retrieved 29 November 2022<br \/>\n                                                    from https:\/\/phys.org\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2022-11-bioreactor-cell-culture-conditions.html<\/p>\n<p>                                             This document is subject to copyright. 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Credit: \u00a9 2022 KAUST; Anastasia Serin A cell-culturing technique developed by KAUST scientists helps to create biological conditions that more closely mirror&#8230;<\/p>\n","protected":false},"author":1,"featured_media":517896,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2022\/bioreactor-keeps-cell-1.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-517895","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/517895","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=517895"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/517895\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/517896"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=517895"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=517895"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=517895"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}