{"id":542501,"date":"2023-01-23T12:31:06","date_gmt":"2023-01-23T09:31:06","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/gene-editing-halts-damage-in-mice-after-heart-attacks-in-new-study\/"},"modified":"2023-01-23T12:31:06","modified_gmt":"2023-01-23T09:31:06","slug":"gene-editing-halts-damage-in-mice-after-heart-attacks-in-new-study","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/gene-editing-halts-damage-in-mice-after-heart-attacks-in-new-study\/","title":{"rendered":"#Gene editing halts damage in mice after heart attacks in new study"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a2d0f8d5d697\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a2d0f8d5d697\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/gene-editing-halts-damage-in-mice-after-heart-attacks-in-new-study\/#%E2%80%9CGene_editing_halts_damage_in_mice_after_heart_attacks_in_new_study%E2%80%9D\" >&#8220;Gene editing halts damage in mice after heart attacks in new study&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CGene_editing_halts_damage_in_mice_after_heart_attacks_in_new_study%E2%80%9D\"><\/span>&#8220;Gene editing halts damage in mice after heart attacks in new study&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2023\/gene-editing-halts-dam.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/2023\/gene-editing-halts-dam.jpg\" data-sub-html=\"The figure shows cross-sections of mouse hearts with areas of damage in red. Treatment with virus-expressing CRISPR components reduces cardiac damage following ischemic injury. Credit: UT Southwestern\">\n<figure class=\"article-img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2023\/gene-editing-halts-dam.jpg\" alt=\"Gene editing halts damage in mice after heart attacks in new study\" title=\"The figure shows cross-sections of mouse hearts with areas of damage in red. Treatment with virus-expressing CRISPR components reduces cardiac damage following ischemic injury. Credit: UT Southwestern\" width=\"600\" height=\"205\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                The figure shows cross-sections of mouse hearts with areas of damage in red. Treatment with virus-expressing CRISPR components reduces cardiac damage following ischemic injury. Credit: UT Southwestern<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>Editing a gene that prompts a cascade of damage after a heart attack <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">app<\/a>eared to reverse this inevitable course in mice, leaving their hearts remarkably unharmed, a new study by UT Southwestern scientists showed. The findings, published in <i><a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">Science<\/a><\/i>, could lead to a new strategy for protecting patients from the consequences of heart disease.<\/p>\n<section class=\"article-banner first-banner ads-336x280\"><!-- \/4988204\/Phys_Story_InText_Box --><br \/>\n      <\/section>\n<p>&#8220;Usually, depriving the heart of oxygen for an extended period, as often happens in a heart attack, will damage it substantially. But those animals whose heart muscles were subjected to gene editing after induced heart attacks seem to be essentially normal in the weeks and months afterward,&#8221; said Eric Olson, Ph.D., Director of the Hamon Center for Regenerative Science and Medicine and Chair of Molecular Biology at UTSW, who co-led the study with Rhonda Bassel-Duby, Ph.D., Professor of Molecular Biology.<\/p>\n<p>Since its discovery a decade ago, the CRISPR-Cas9 gene editing system has been used by scientists to correct genetic mutations responsible for disease, including work by the Olson lab on Duchenne muscular dystrophy. However, Dr. Bassel-Duby explained, these diseases caused by mutations affect relatively small groups of people, whereas nongenetic diseases affect far larger numbers. For example, cardiovascular diseases are the leading cause of death globally, killing about 19 million people every year.<\/p>\n<p>Researchers recently discovered that much of the damage from a heart attack\u2014an event characterized by blockage of blood vessels that feed the heart, depriving it of oxygen\u2014is caused by overactivation of a gene called <i>CaMKII\u03b4. <\/i>This gene plays a variety of roles in heart cell signaling and function. The overactivation occurs when the heart is stressed, prompted by oxidation of two methionine amino acids that form part of the <i>CaMKII\u03b4<\/i> protein.<\/p>\n<p>Drs. Olson and Bassel-Duby and their colleagues reasoned that if these methionines could be converted to a different amino acid instead, oxidation wouldn&#8217;t occur, sparing the heart from <i>CaMKII\u03b4<\/i> overactivation and subsequent damage after a heart attack.<\/p>\n<p>To test this idea, Simon Lebek, M.D., a postdoctoral fellow, and other members of the team used CRISPR-Cas9 to edit <i>CaMKII\u03b4<\/i> in human heart cells growing in a petri dish. Tests showed that when unedited heart cells were placed into a low-oxygen chamber, they developed numerous markers of damage and subsequently died. However, the edited cells were protected from damage and survived.<\/p>\n<p>The researchers then tried a similar experiment in live mice, inducing a heart attack in these animals by restricting blood flow to their heart&#8217;s main pumping chamber for 45 minutes and then delivering <i>CaMKII\u03b4<\/i> gene editing components directly to some animals&#8217; hearts. Both mice that received gene editing and those that did not had severely compromised heart function in the first 24 hours after their heart attacks. But while the mice without the gene editing continued to worsen over time, those that received gene editing steadily improved over the next few weeks, ultimately achieving cardiac function that was nearly indistinguishable from before their heart attacks.<\/p>\n<p>Further research showed that the gene editing appeared to be isolated to the heart\u2014there was no evidence of edited <i>CaMKII\u03b4<\/i> in other organs, including the liver, brain, or muscles. No negative side effects were apparent almost a year out from treatment, Drs. Olson and Bassel-Duby said. The treatment also appeared to be durable, they added, noting that the gene-edited mice were able to do heavy exercise similar to mice that never had heart attacks.<\/p>\n<p>Although this treatment will need substantial safety and efficacy studies before it can be used in humans, the researchers suggest that gene editing could offer a promising solution for treating patients in the aftermath of a heart attack and could have potential for a range of other nongenetic diseases.<\/p>\n<p>&#8220;Rather than targeting a genetic mutation, we essentially modified a normal gene to make sure it wouldn&#8217;t become harmfully overactive. It&#8217;s a new way of using CRISPR-Cas9 gene editing,&#8221; Dr. Bassel-Duby said.<\/p>\n<p>Dr. Olson holds the Pogue Distinguished Chair in Research on Cardiac Birth Defects, The Robert A. Welch Distinguished Chair in Science, and the Annie and Willie Nelson Professorship in Stem Cell Research.<\/p>\n<p>Other UTSW researchers who contributed to this study include Francesco Chemello, Xurde M. Caravia, Wei Tan, Hui Li, Kenian Chen, Lin Xu, and Ning Liu.<\/p>\n<div class=\"article-main__more p-4\">\n                                                                                        <strong>More information:<\/strong><br \/>\n                                                Simon Lebek et al, Ablation of CaMKII\u03b4 oxidation by CRISPR-Cas9 base editing as a therapy for cardiac disease, <i>Science<\/i> (2023).  <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.1126\/science.ade1105\">DOI: 10.1126\/science.ade1105<\/a><\/p><\/div>\n<div class=\"d-inline-block text-medium mt-4\">\n<p>                                                    Provided by<br \/>\n                                                                                                            UT Southwestern Medical Center<br \/>\n                                                                                                                <a rel=\"nofollow noopener\" target=\"_blank\" class=\"icon_open\" href=\"http:\/\/www8.utsouthwestern.edu\/index.html\"><br \/>\n                                                            <svg><use href=\"https:\/\/phys.b-cdn.net\/tmpl\/v6\/img\/svg\/sprite.svg#icon_open\" x=\"0\" y=\"0\"\/><\/svg><\/a><\/p><\/div>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>                                                <strong>Citation<\/strong>:<br \/>\n                                                Gene editing halts damage in mice after heart attacks in new study (2023, January 23)<br \/>\n                                                retrieved 23 January 2023<br \/>\n                                                from https:\/\/phys.org\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2023-01-gene-halts-mice-heart.html<\/p>\n<p>                                             This document is subject to copyright. 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Treatment with virus-expressing CRISPR components reduces cardiac damage following ischemic injury. Credit: UT Southwestern Editing a gene that prompts a cascade of damage after a heart attack appeared to reverse&#8230;<\/p>\n","protected":false},"author":1,"featured_media":542502,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/2023\/gene-editing-halts-dam.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-542501","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/542501","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=542501"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/542501\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/542502"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=542501"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=542501"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=542501"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}