{"id":548379,"date":"2023-02-06T14:00:02","date_gmt":"2023-02-06T11:00:02","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/new-insights-into-centromere-structure\/"},"modified":"2023-02-06T14:00:02","modified_gmt":"2023-02-06T11:00:02","slug":"new-insights-into-centromere-structure","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/new-insights-into-centromere-structure\/","title":{"rendered":"#New insights into centromere structure"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a23b11dc5f28\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a23b11dc5f28\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-1'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/new-insights-into-centromere-structure\/#%E2%80%9CNew_insights_into_centromere_structure%E2%80%9D\" >&#8220;New insights into centromere structure&#8221;<\/a><\/li><\/ul><\/nav><\/div>\n<h1><span class=\"ez-toc-section\" id=\"%E2%80%9CNew_insights_into_centromere_structure%E2%80%9D\"><\/span>&#8220;New insights into centromere structure&#8221;<span class=\"ez-toc-section-end\"><\/span><\/h1>\n<div>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2023\/new-insights-into-cent.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/2023\/new-insights-into-cent.jpg\" data-sub-html=\"Cryo EM structure of the CENP-A nucleosome complex with KNL2. Credit: Jiang et al., EMBO J., 2023\">\n<figure class=\"article-img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2023\/new-insights-into-cent.jpg\" alt=\"New insights into centromere structure\" title=\"Cryo EM structure of the CENP-A nucleosome complex with KNL2. Credit: Jiang et al., EMBO J., 2023\" width=\"800\" height=\"440\"\/><figcaption class=\"text-darken text-low-up text-truncate-js text-truncate mt-3\">\n                Cryo EM structure of the CENP-A nucleosome complex with KNL2. Credit: Jiang et al., EMBO J., 2023<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>Researchers led by Osaka University used cryogenic electron microscopy to analyze the atomic structure of the centromeric region of the chromosome, essential for cell division. A protein called CENP-A marks the centromere; the researchers showed that during interphase, CENP-A is bound by a protein called KNL2 to maintain the location of the centromere. During mitosis, KNL2 is replaced by CENP-C, allowing correct formation of the kinetochore complex for cell division.<\/p>\n<section class=\"article-banner first-banner ads-336x280\"><!-- \/4988204\/Phys_Story_InText_Box --><br \/>\n      <\/section>\n<p>The genetic material inside cells is organized into structures called chromosomes. The centromere is essential for the correct division of the chromosomes via interaction with spindle microtubules when cells divide and grow. Now, a study by researchers at Osaka University has clarified the structure of the centromeric region in chicken cells using a technique known as cryogenic electron microscopy (cryo-EM).<\/p>\n<p>Cryo-EM freezes samples quickly to preserve and stabilize them, and then images them using collisions with electrons to reveal their structure. A complex of proteins called the &#8220;kinetochore&#8221; forms at the centromeric region, and this is essential for cells to divide correctly. The researchers were able to clarify a structural change to the kinetochore at the atomic level using cryo-EM analysis.<\/p>\n<p>When DNA is condensed into chromosomes, it is coiled around a core made of proteins called histones to form a structure known as a nucleosome. The nucleosomes in the centromeric region contain a variant histone protein called CENP-A, which specifies the location of the centromere. However, the mechanisms by which CENP-A is deposited at the centromeres to correctly define their location were unknown until now.\n<\/p>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2023\/new-insights-into-cent-1.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2023\/new-insights-into-cent-1.jpg\" data-sub-html=\"KNL2 binds to the CENP-A nucleosome during interphase and it contributes to new CENP-A deposition into centromeres via HJURP and the Mis18 complex. Credit: Original content by Tatsuo Fukagawa\">\n<figure class=\"article-img text-center\"><img decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2023\/new-insights-into-cent-1.jpg\" alt=\"New insights into centromere structure\" title=\"KNL2 binds to the CENP-A nucleosome during interphase and it contributes to new CENP-A deposition into centromeres via HJURP and the Mis18 complex. Credit: Original content by Tatsuo Fukagawa\"\/><figcaption class=\"text-left text-darken text-truncate text-low-up mt-3\">\n                KNL2 binds to the CENP-A nucleosome during interphase and it contributes to new CENP-A deposition into centromeres via HJURP and the Mis18 complex. Credit: Original content by Tatsuo Fukagawa<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>The research team showed that during mitosis, a protein called CENP-C binds CENP-A and acts as a scaffold for other kinetochore proteins. However, during interphase (the time when the cell is not dividing), a different protein called KNL2 binds to centromeres instead. &#8220;KNL2 contains a CENP-C-like motif and is a component of the Mis18 complex, a licensing factor for new CENP-A deposition,&#8221; explains lead authors of the study Honghui Jiang and Mariko Ariyoshi.<\/p>\n<p>The team further revealed that this interaction between KNL2 and the centromere is required for new deposition of CENP-A during interphase, which in turn helps maintain the correct location of the centromere.<\/p>\n<p>&#8220;We also showed that CENP-C is phosphorylated during mitosis, and phosphorylated CENP-C excludes KNL2 from the KNL2\u2013CENP-A complex,&#8221; explains senior author Tatsuo Fukagawa. This suggests that KNL2 binds to CENP-A through interphase, maintaining the location of the centromere until a phosphate molecule becomes bound to CENP-C as the cells reach mitosis. Then, CENP-C preferentially binds to CENP-A, allowing the formation of the kinetochore for cell division.\n<\/p>\n<div class=\"article-gallery lightGallery\">\n<div data-thumb=\"https:\/\/scx1.b-cdn.net\/csz\/news\/tmb\/2023\/new-insights-into-cent-2.jpg\" data-src=\"https:\/\/scx2.b-cdn.net\/gfx\/news\/hires\/2023\/new-insights-into-cent-2.jpg\" data-sub-html=\"CENP-C excludes KNL2 from the CENP-A-KNL2 complex during mitosis. Credit: Original content by Tatsuo Fukagawa\">\n<figure class=\"article-img text-center\"><img decoding=\"async\" src=\"https:\/\/scx1.b-cdn.net\/csz\/news\/800a\/2023\/new-insights-into-cent-2.jpg\" alt=\"New insights into centromere structure\" title=\"CENP-C excludes KNL2 from the CENP-A-KNL2 complex during mitosis. Credit: Original content by Tatsuo Fukagawa\"\/><figcaption class=\"text-left text-darken text-truncate text-low-up mt-3\">\n                CENP-C excludes KNL2 from the CENP-A-KNL2 complex during mitosis. Credit: Original content by Tatsuo Fukagawa<br \/>\n            <\/figcaption><\/figure>\n<\/div>\n<\/div>\n<p>These new insights into the structure of the centromeric region will prove invaluable in advancing knowledge of cell division and growth. Proteins involved in cell division and the kinetochore are targets for anti-cancer drugs; therefore, this work will also contribute to the design of novel drugs for diseases such as cancer.<\/p>\n<p>The research is published in <i>The EMBO Journal<\/i>.<\/p>\n<div class=\"article-main__more p-4\">\n<p><strong>More information:<\/strong><br \/>\n                                                Honghui Jiang et al, The cryo-EM structure of the CENP-A nucleosome in complex with ggKNL2, <i>The EMBO Journal<\/i> (2023). <a rel=\"nofollow noopener\" target=\"_blank\" data-doi=\"1\" href=\"https:\/\/dx.doi.org\/10.15252\/embj.2022111965\">DOI: 10.15252\/embj.2022111965<\/a><\/p><\/div>\n<div class=\"d-inline-block text-medium mt-4\">\n<p>                                                    Provided by<br \/>\n                                                                                                            Osaka University<br \/>\n                                                                                                                <a rel=\"nofollow noopener\" target=\"_blank\" class=\"icon_open\" href=\"http:\/\/www.osaka-u.ac.jp\/en\"><br \/>\n                                                            <svg><use href=\"https:\/\/phys.b-cdn.net\/tmpl\/v6\/img\/svg\/sprite.svg#icon_open\" x=\"0\" y=\"0\"\/><\/svg><\/a><\/p><\/div>\n<p>                                        <!-- print only --><\/p>\n<div class=\"d-none d-print-block\">\n<p>                                                <strong>Citation<\/strong>:<br \/>\n                                                New insights into centromere structure (2023, February 6)<br \/>\n                                                retrieved 6 February 2023<br \/>\n                                                from https:\/\/phys.org\/<a href=\"https:\/\/buradabiliyorum.com\/en\/category\/news\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"News\" target=\"_blank\" rel=\"noopener\">news<\/a>\/2023-02-insights-centromere.html<\/p>\n<p>                                             This document is subject to copyright. 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Credit: Jiang et al., EMBO J., 2023 Researchers led by Osaka University used cryogenic electron microscopy to analyze the atomic structure of the centromeric region of the chromosome, essential for cell division. A protein called CENP-A marks the centromere; the&#8230;<\/p>\n","protected":false},"author":1,"featured_media":548380,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/scx2.b-cdn.net\/gfx\/news\/2023\/new-insights-into-cent.jpg","fifu_image_alt":"","footnotes":""},"categories":[16],"tags":[],"class_list":["post-548379","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sciencee"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/548379","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=548379"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/548379\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/548380"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=548379"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=548379"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=548379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}