{"id":617882,"date":"2024-04-22T19:08:02","date_gmt":"2024-04-22T16:08:02","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/new-super-resolution-microscopy-approach-visualizes-internal-cell-structures-and-clusters-via-selective-plane-activation\/"},"modified":"2024-04-22T19:08:02","modified_gmt":"2024-04-22T16:08:02","slug":"new-super-resolution-microscopy-approach-visualizes-internal-cell-structures-and-clusters-via-selective-plane-activation","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/new-super-resolution-microscopy-approach-visualizes-internal-cell-structures-and-clusters-via-selective-plane-activation\/","title":{"rendered":"#New super-resolution microscopy approach visualizes internal cell structures and clusters via selective plane activation"},"content":{"rendered":"
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\n Fluorescence images of nuclei in a cell spheroid labeled by rsGamillus-S with a diameter of 100 \u00b5m observed with widefield, SPA-SIM, and 3DSIM at a depth of 43 \u00b5m. Credit: From Nature Methods<\/i> (2024). DOI: 10.1038\/s41592-024-02236-3
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To study living organisms at ever smaller length scales, scientists must devise new techniques to overcome the so-called diffraction limit. This is the intrinsic limitation on a microscope’s ability to focus on objects smaller than the wavelength of light being used.<\/p>\n


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Structured illumination microscopy is one of the super-resolution techniques that can help, by illuminating sinusoidal-patterned light on a sample to obtain a sharper image. However, background light from out-of-focus regions can still smear out the final picture.<\/p>\n

In a study recently published<\/a> in the journal Nature Methods<\/i>, researchers from Osaka University demonstrated a new app<\/a>roach for super-resolution microscopy capable of observing structures inside a single cell or a cell cluster. This was accomplished by selecting only a desired plane to image using thin “light sheet” illumination, projected perpendicular to the lens, to switch on fluorophores.<\/p>\n

“We show that selective-plane activation allows us to image dense microstructures inside cells with excellent sharpness not readily available previously,” lead author of the study, Kenta Temma says. That is, sinusoidal “structured” light selectively excited only a thin plane where on-state fluorophores were localized, which allowed for background-free super-resolution imaging.<\/p>\n