{"id":84427,"date":"2020-10-08T10:47:34","date_gmt":"2020-10-08T07:47:34","guid":{"rendered":"https:\/\/en.buradabiliyorum.com\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/"},"modified":"2020-10-08T10:47:34","modified_gmt":"2020-10-08T07:47:34","slug":"engineer-explains-how-nobel-prize-winning-crispr-technique-works","status":"publish","type":"post","link":"https:\/\/buradabiliyorum.com\/en\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/","title":{"rendered":"#Engineer explains how Nobel Prize-winning CRISPR technique works"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<label for=\"ez-toc-cssicon-toggle-item-6a26bb76d82a6\" class=\"ez-toc-cssicon-toggle-label\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #dd3333;color:#dd3333\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #dd3333;color:#dd3333\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/label><input type=\"checkbox\"  id=\"ez-toc-cssicon-toggle-item-6a26bb76d82a6\" checked aria-label=\"Toggle\" \/><nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/buradabiliyorum.com\/en\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/#CRISPRCas_systems_as_gene_editors\" >CRISPR\/Cas systems as gene editors<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/buradabiliyorum.com\/en\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/#Why_CRISPR_deserved_a_Nobel_Prize\" >Why CRISPR deserved a Nobel Prize<\/a><\/li><\/ul><\/nav><\/div>\n<p>&#8220;<strong>#Engineer explains how Nobel Prize-winning CRISPR technique works<\/strong>&#8221;<\/p>\n<div>\n                            Researchers have been able to manipulate large chunks of genetic code for almost 50 years. But it is only within the past decade that they have been able to do it with exquisite precision \u2013 adding, deleting and substituting single units of the genetic code just as an editor can manipulate a single letter in a document. This newfound ability is called gene-editing, the tool is called CRISPR, and it\u2019s being used worldwide to engineer plants and livestock and treat disease in people.<\/p>\n<p>For these reasons the <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.nobelprize.org\/prizes\/chemistry\/2020\/press-release\/\">2020 Nobel Prize in chemistry<\/a> has been awarded to <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/scholar.google.fr\/citations?user=fZJ8R-QAAAAJ&amp;hl=fr\">Emmanuelle Charpentier<\/a>, director of the Max Planck Unit for the <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/sciencee\/\" data-internallinksmanager029f6b8e52c=\"5\" title=\"Science\" target=\"_blank\" rel=\"noopener\">Science<\/a> of Pathogens in Germany, and <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/scholar.google.com\/citations?user=YO5XSXwAAAAJ&amp;hl=en\">Jennifer Doudna<\/a>, professor at the University of California, Berkeley, for discovering and transforming CRISPR into a gene-editing <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/technology\/\" data-internallinksmanager029f6b8e52c=\"4\" title=\"Technology\" target=\"_blank\" rel=\"noopener\">technology<\/a>. It\u2019s the first time two women have shared a Nobel prize.<\/p>\n<p><a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.che.ufl.edu\/jain\/\">I\u2019m a CRISPR engineer<\/a>, interested in developing novel CRISPR-based gene-editing tools and delivery methods to improve their precision and function.<\/p>\n<p>In the past, my colleagues and I have created a version of CRISPR that can be <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1002\/anie.201606123\">controled using light<\/a>, which allows precise control of where and when gene editing is performed in cells, and can be potentially used in animals and humans. We\u2019ve also created <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1039\/C9NR01786K\">a targeted system<\/a> that can package and deliver the editing components to desirable cell types \u2013 it\u2019s like GPS for cells. Most recently, we engineered a tool <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1038\/s41467-020-18615-1\">that improved the speed and precision of CRISPR<\/a> so it could be used in rapid diagnostic kits for <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/rapid-home-based-coronavirus-tests-are-coming-together-in-research-labs-were-working-on-analyzing-spit-using-advanced-crispr-gene-editing-techniques-138064\">COVID-19, HIV, HCV and prostate cancer<\/a>.<\/p>\n<p>While CRISPR scientists like me have been speculating about a Nobel Prize for CRISPR, it was exciting to see Charpentier and Doudna win. This will encourage young, talented engineers and researchers to enter the field of gene editing, which can be leveraged for designing new diagnostics, treatments and cures for a range of diseases.<\/p>\n<figure class=\"align-center \">\n<figure class=\"post-image post-mediaBleed aligncenter\"><img loading=\"lazy\" decoding=\"async\" sizes=\"auto, (min-width: 1466px) 754px, (max-width: 599px) 100vw, (min-width: 600px) 600px, 237px\" alt=\"\" width=\"600\" height=\"300\" class=\" lazy\" src=\"https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;fit=clip\" data-lazy=\"true\" srcset=\"https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=600&amp;h=300&amp;fit=crop&amp;dpr=1 600w, https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=600&amp;h=300&amp;fit=crop&amp;dpr=2 1200w, https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=600&amp;h=300&amp;fit=crop&amp;dpr=3 1800w, https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;h=377&amp;fit=crop&amp;dpr=1 754w, https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=754&amp;h=377&amp;fit=crop&amp;dpr=2 1508w, https:\/\/images.theconversation.com\/files\/362274\/original\/file-20201007-22-xl6nvl.jpg?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=754&amp;h=377&amp;fit=crop&amp;dpr=3 2262w\"\/><figcaption><a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/thenextweb.com\/syndication\/2020\/10\/08\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/#\" data-url=\"https:\/\/twitter.com\/intent\/tweet?url=https%3A%2F%2Fthenextweb.com%2Fsyndication%2F2020%2F10%2F08%2Fengineer-explains-how-nobel-prize-winning-crispr-technique-works%2F&amp;via=thenextweb&amp;related=thenextweb&amp;text=Check out this picture on: Gene-editing technology enables researchers to edit the DNA of organisms and reprogram them. Johan Jarnestad\/The Royal Swedish Academy of Sciences, CC BY-NC\" data-title=\"Share Gene-editing technology enables researchers to edit the DNA of organisms and reprogram them. Johan Jarnestad\/The Royal Swedish Academy of Sciences, CC BY-NC on Twitter\" data-width=\"685\" data-height=\"500\" class=\"post-image-share popitup\" title=\"Share Gene-editing technology enables researchers to edit the DNA of organisms and reprogram them. Johan Jarnestad\/The Royal Swedish Academy of Sciences, CC BY-NC on Twitter\"><i class=\"icon icon--inline icon--twitter--dark\"\/><\/a>Gene-editing technology enables researchers to edit the DNA of organisms and reprogram them. Johan Jarnestad\/The Royal Swedish Academy of Sciences, CC BY-NC<\/figcaption><\/figure>\n<\/p>\n<\/figure>\n<h2><span class=\"ez-toc-section\" id=\"CRISPRCas_systems_as_gene_editors\"><\/span>CRISPR\/Cas systems as gene editors<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Many variants of CRISPR\/Cas systems have been discovered, engineered and <a href=\"https:\/\/buradabiliyorum.com\/en\/category\/download-scripts-themes-apps\/\" data-internallinksmanager029f6b8e52c=\"9\" title=\"Download Scripts &amp; Themes &amp; Apps\" target=\"_blank\" rel=\"noopener\">app<\/a>lied to edit genes. There are already over <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=%22CRISPR%22\">20,000 scientific publications<\/a> on the topic.<\/p>\n<p>CRISPR dates back to 1987, when a Japanese molecular biologist, Yoshizumi Ishino, and colleagues discovered <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1128\/jb.169.12.5429-5433.1987\">a CRISPR DNA sequence<\/a> in <em>E. coli<\/em>. The CRISPR sequence was later characterized by a Spanish scientist, Francisco Mojica, and colleagues, who named it CRISPR, which stands for <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1046\/j.1365-2958.2002.02839.x\">Clustered Regularly Interspaced Short Palindromic Repeats<\/a>.<\/p>\n<p>While people and animals have evolved complex immune systems to fight viral attacks, single-cell microorganisms rely on CRISPR to find and destroy a virus\u2019s genetic material to stop it from multiplying.<\/p>\n<p>Charpentier and Doudna figured out how to borrow this innate biological capability from microbes and apply it to genetic engineering of bacteria.<\/p>\n<p>In a landmark paper, published online on June 28, 2012, Charpentier and Doudna showed that the CRISPR gene-editing machinery includes two components: a guide molecule that serves as sort of a GPS to find and bind the target gene site on the DNA of an invading virus, which then teams up with a CRISPR-associated protein (Cas) that serves as a molecular scissor <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1126\/science.1225829\">that snips the DNA<\/a>.<\/p>\n<figure><iframe loading=\"lazy\" title=\"How CRISPR lets us edit our DNA | Jennifer Doudna\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/TdBAHexVYzc?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/figure>\n<p>Around the same time, <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.researchgate.net\/scientific-contributions\/39194791-Virginijus-Siksnys\">Virginijus Siksnys<\/a>, a Lithuanian biochemist at the University of Vilnius, made a similar discovery and submitted results for publication that appeared a few months later, in <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.pnas.org\/content\/109\/39\/E2579\">September 2012<\/a>. <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/scholar.google.com\/citations?user=B5QpZooAAAAJ&amp;hl=en\">Feng Zhang<\/a>, a biologist at the Broad Institute in Cambridge, Massachusetts, and colleagues showed that CRISPR can be improved and used for <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1126\/science.1231143\">editing mammalian cells<\/a>. He currently owns one of the first patents on using CRISPR for gene editing, which is being <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.sciencemag.org\/news\/2020\/09\/latest-round-crispr-patent-battle-has-apparent-victor-fight-continues\">contested<\/a> by Doudna\u2019s institution, UC Berkeley.<\/p>\n<p>Once the DNA has been cut in the right spot, the cell will try to repair the cut. But the repair mechanism is error prone, and oftentimes the cells fail to fix the cuts perfectly, ultimately disabling the gene. Disrupting a gene is particularly useful for studying its function and find out what happens if you stop a gene from working. This technique is also useful for treating cancer and infections, where turning off a gene can potentially stop cancer cells and pathogens from dividing or kill them outright.<\/p>\n<p>During this cutting-repair process, one can fool the cells by providing a new piece of DNA. The cells will then incorporate this piece of DNA with desirable edits into the genetic code. This enables researchers to correct a genetic mutation that causes a genetic disease, or replace a defective gene with a healthy one.<\/p>\n<p>The beauty of CRISPR lies in its simplicity. CRISPR can be easily customized to target any gene of interest, whether it is in plants, animals or people. CRISPR applications range from tools for understanding biology, as diagnostics and <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/doi.org\/10.1038\/s41571-019-0166-8\">as new kinds of therapeutics<\/a> to <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"http:\/\/doi.org\/10.1016\/j.cell.2014.05.010\">applications in producing better crops, biofuels and transplantable organs<\/a>.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Why_CRISPR_deserved_a_Nobel_Prize\"><\/span>Why CRISPR deserved a Nobel Prize<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>While there is still plenty of room for improvement of these technologies, scientists have already begun testing CRISPR in a number of <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/clinicaltrials.gov\/ct2\/results?cond=&amp;term=CRISPR&amp;cntry=&amp;state=&amp;city=&amp;dist=\">clinical trials<\/a> for treating cancer and genetic disorders. CRISPR-based diagnostics have been also been approved by the U.S. Food and Drug Administration under emergency use authorization for <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.nature.com\/articles\/d41586-020-01402-9\">COVID-19 testing<\/a>.<\/p>\n<p>CRISPR does come with a lot of ethical concerns that warrant caution. For example, in 2018, a Chinese scientist prematurely and unethically used CRISPR for editing human embryos and created <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/us\/topics\/crispr-edited-baby-63150\">CRISPR-edited babies<\/a> that could pass these genetic alterations to their offspring for generations to come. Some have used the technology for <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.popularmechanics.com\/science\/a19067\/11-crazy-things-we-can-do-with-crispr-cas9\/\">other CRISPR-related DIY biohacks<\/a> that raise more <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/www.vox.com\/future-perfect\/2019\/8\/13\/20802059\/california-crispr-biohacking-illegal-josiah-zayner\">concerns over regulating the gene-editing technology<\/a>.<\/p>\n<p>Despite these concerns, CRISPR has huge potential to transform how scientists can <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/rapid-home-based-coronavirus-tests-are-coming-together-in-research-labs-were-working-on-analyzing-spit-using-advanced-crispr-gene-editing-techniques-138064\">detect<\/a>, <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/how-gene-edited-white-blood-cells-are-helping-fight-cancer-126806\">treat<\/a> and even <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/using-gene-drives-to-control-wild-mosquito-populations-and-wipe-out-malaria-104613\">eradicate diseases<\/a> as well as improve agricultural products. Society is already seeing the benefits of this Nobel-winning technology.<!-- Below is The Conversation's page counter tag. Please DO NOT REMOVE. --><img loading=\"lazy\" decoding=\"async\" style=\"border: none !important; box-shadow: none !important; margin: 0 !important; max-height: 1px !important; max-width: 1px !important; min-height: 1px !important; min-width: 1px !important; opacity: 0 !important; outline: none !important; padding: 0 !important; text-shadow: none !important;\" alt=\"The Conversation\" width=\"1\" height=\"1\" class=\" lazy\" src=\"https:\/\/counter.theconversation.com\/content\/147701\/count.gif?distributor=republish-lightbox-basic\" data-lazy=\"true\"\/><!-- End of code. If you don't see any code above, please get new code from the Advanced tab after you click the republish button. The page counter does not collect any personal data. More info: https:\/\/theconversation.com\/republishing-guidelines --><\/p>\n<p><em>This article is republished from <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\">The Conversation<\/a>\u00a0by\u00a0<a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/profiles\/piyush-k-jain-884131\">Piyush K. Jain<\/a>, Assistant Professor of Chemical Engineering, Herbert Wertheim College of Engineering, UF Health Cancer Center, <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/institutions\/university-of-florida-1392\">University of Florida<\/a>\u00a0under a Creative Commons license. Read the <a rel=\"nofollow noopener noreferrer\" target=\"_blank\" href=\"https:\/\/theconversation.com\/nobel-prize-for-chemistry-honors-exquisitely-precise-gene-editing-technique-crispr-a-gene-engineer-explains-how-it-works-147701\">original article<\/a>.<\/em><\/p>\n<\/p><\/div>\n<p><script async src=\"\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<blockquote>\n<p style=\"text-align: center;\">For forums sites go to <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/forum.buradabiliyorum.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Forum.BuradaBiliyorum.Com<\/a><\/span><\/strong>\n<\/p><\/blockquote>\n<blockquote>\n<p style=\"text-align: center;\"><strong>If you want to read more like this article, you can visit our <span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/en.buradabiliyorum.com\/technology\/\" target=\"_blank\" rel=\"noopener noreferrer\">Technology category.<\/a><\/span><\/strong><\/p>\n<\/blockquote>\n<p><span style=\"color: black;\"><a style=\"color: #ff9900;\" href=\"https:\/\/thenextweb.com\/syndication\/2020\/10\/08\/engineer-explains-how-nobel-prize-winning-crispr-technique-works\/\" target=\"_blank\" rel=\"noopener noreferrer\">Source<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;#Engineer explains how Nobel Prize-winning CRISPR technique works&#8221; Researchers have been able to manipulate large chunks of genetic code for almost 50 years. But it is only within the past decade that they have been able to do it with exquisite precision \u2013 adding, deleting and substituting single units of the genetic code just as&#8230;<\/p>\n","protected":false},"author":1,"featured_media":84428,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/img-cdn.tnwcdn.com\/image\/tnw?filter_last=1&fit=1280,640&url=https:\/\/cdn0.tnwcdn.com\/wp-content\/blogs.dir\/1\/files\/2020\/10\/image-7-2.png&signature=791dc636bf730386f4749085c332c782","fifu_image_alt":"","footnotes":""},"categories":[18],"tags":[74850,74849,74851,74364,73644,70759],"class_list":["post-84427","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-technology","tag-crispr","tag-gene-editing","tag-genetic","tag-nobel-prize","tag-scientist","tag-tech"],"_links":{"self":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/84427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/comments?post=84427"}],"version-history":[{"count":0,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/posts\/84427\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media\/84428"}],"wp:attachment":[{"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/media?parent=84427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/categories?post=84427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/buradabiliyorum.com\/en\/wp-json\/wp\/v2\/tags?post=84427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}