#Safer CRISPR gene editing with fewer off-target hits

“#Safer CRISPR gene editing with fewer off-target hits” Mutating the enzyme at the heart of the CRISPR gene editing system can improve its fidelity. Credit: DataBase Center for Life Science (DBCLS) The CRISPR system is a powerful tool for the targeted editing of genomes, with significant therapeutic potential, but runs the risk of inappropriately editing…

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#How to precisely edit mitochondrial DNA

“#How to precisely edit mitochondrial DNA” While precisely editing the DNA in a cell’s nucleus (blue) has become easier thanks to advances like CRISPR/Cas-9, it’s been much harder to edit DNA in the mitochondria (red). Now, a new tool brings precision gene editing to mitochondrial DNA. Credit: Tslil Ast/Mootha Lab Scientists can now precisely edit…

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#CRISPR enables one-step hybrid seed production in crops

“#CRISPR enables one-step hybrid seed production in crops” An ear of an MGM maintainer plant was photographed under a bright field (left panel) and under 554 nm wavelength excitation with a filter (right panel). The red fluorescent kernels (one example indicated by the blue arrow in the right panel) were MGM maintainer seeds The kernels…

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#Researchers design artificial genes to sense cellular responses to drugs

“#Researchers design artificial genes to sense cellular responses to drugs” Credit: CC0 Public Domain Researchers from Boston University School of Medicine (BUSM) have developed and implemented a new way to better understand how human cells communicate with each other, how this communication is disrupted in human diseases and how this can be corrected pharmacologically. Their…

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#Anaplasmosis bacterium tinkers with tick’s gene expression to spread to new hosts

“#Anaplasmosis bacterium tinkers with tick’s gene expression to spread to new hosts” Anaplasmosis bacterium tinkers with tick’s gene expression to spread to new hosts. Credit: Erik Karits, Pixabay For the first time, scientists have shown that the bacterium that causes the tick-borne disease anaplasmosis interferes with tick gene expression for its survival inside cells and…

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#New candidate for raw material synthesis through gene transfer

“#New candidate for raw material synthesis through gene transfer” Tilman Lamparter is Professor of General Botany at the KIT. Credit: Amadeus Bramsiepe, KIT Cyanobacteria hardly need any nutrients and use the energy of sunlight. Bathers are familiar with these microorganisms—often incorrectly called “blue-green algae”—as they often occur in waters. A group of researchers at the…

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#Tiny mineral particles are better vehicles for promising gene therapy

“#Tiny mineral particles are better vehicles for promising gene therapy” DNA, which has a double-helix structure, can have many genetic mutations and variations. Credit: NIH University of Wisconsin–Madison researchers have developed a safer and more efficient way to deliver a promising new method for treating cancer and liver disorders and for vaccination—including a COVID-19 vaccine…

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#CRISPR-assisted novel method detects RNA-binding proteins in living cells

“#CRISPR-assisted novel method detects RNA-binding proteins in living cells” CARPID is applied to three different lncRNAs. The result shows that there was not much overlapping of binding proteins, which demonstrated the high specificity of CARPID. Credit: Nature Methods (2020). DOI: 10.1038/s41592-020-0866-0 Two steps of CARPID: navigation and biotin-labeling To overcome the limitations of the existing…

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#The mystery of pollen sterility and its reversion in pigeon pea revealed in a new study

“#The mystery of pollen sterility and its reversion in pigeon pea revealed in a new study” The Vienna Metabolomics Centre (VIME), University of Vienna, in collaboration with International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), based in India has made a breakthrough in pigeonpea by resolving the mystery behind fertility-sterility transition in pigeonpea. Credit:…

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